To investigate whether vlPAGCaMKIIα+ neurons regulate chronic neuropathic-induced anxiety, we injected a Cre-dependent activation virus (AAV2/9-CamKIIα-hM3Dq-mCherry) into the right vlPAG of SNI model mice to specifically activate glutamatergic neurons and evaluate the effects of these neurons on the mechanical pain threshold and anxiety-like behaviors. Here, CAMK2A is linked to Anxiety.