However, ZO‐1 protein expression was observed to be prominent in the periphery of cellular clumps in AlgLung hydrogels suggesting a tighter epithelial barrier (Figure S11, Supporting Information).[29] Such complex, co‐expression of epithelial and mesenchymal markers is not atypical for clinical LUAD samples that was recapitulated in tumor‐mimetic hydrogels.[30] Moreover, sulfated hydrogels strongly induced the expression of several EMT‐regulating transcription factors such as ZEB1, ZEB2, and SNAIL (Figure 3b), further emphasizing the role of ECM sulfation (Figure 3c). This evidence concerns the gene ZEB1 and neoplasm.