The major highlights of this study are as follows: 1) we depicted the expressional feature of FOXK1 in the context of kidney fibrosis progression; 2) we found that FOXK1 aggravates renal fibrosis partly due to its role in triggering glycolysis in proximal TECs; 3) we identified the glycolysis‐related genes regulated by FOXK1 and deciphered the mechanism of LLPS‐mediated transcription, including the corresponding IDR and the subunit of RNA Pol II involved (Figure 8K); 4) we also explored the potential of FOXK1‐targeted gene therapy in CKD treatment. This evidence concerns the gene FOXK1 and chronic kidney disease.