From our analysis results, the 12 genes (MCM4, POLA1, MCM6, MCM3, RBBP4, DNA2, AP2B1, KIF11, KIF23, TUBA1B, KIF14, NUDT21) in HCC tissues have significant expression differences, and also affect the overall survival rate of patients. The gene discussed is MCM4; the disease is hepatocellular carcinoma.