Comparison of the pathway analyses results showed that DNA repair related pathways (DNA repair, mitotic spindle, E2F targets, G2M checkpoint, UV response DN, ROS pathway), UPR, apoptosis, TNFα signaling via NFκB, TGFβ, PI3K/mTOR, and Hedgehog signaling were markedly enriched in the BRAFMT colorectal cancer cell line group compared with the BRAF/KRASWT colorectal cancer cells. The gene discussed is BRAF; the disease is colorectal cancer.