Immunoblot analysis using the antibodies specific for the C-terminus of FER confirmed the fusion protein expression of MAN2A1-FER in all the HCC cell lines positive for the fusion genes while negative for HEP3B cells, HUH7ko cells where MAN2A1-FER expression was disrupted, and a normal liver sample. Here, MAN2A1 is linked to hepatocellular carcinoma.