In this study, we specifically elucidated whether the expression of Nrf2 in MSCs is crucial for leukemia cell migration and invasion, examine if SDF-1/CXCR4 signal axis is activated by Nrf2 overexpression in MSCs, and analyze whether it is the key signal axis between Nrf2 overexpressed MSCs and ALL for promoting leukemia cell invasion, which provides a basis for further exploring the potential downstream molecular mechanisms of interaction between Nrf2-overexpressing MSCs and B-ALL cells. Here, CXCL12 is linked to acute lymphoblastic leukemia.