Likewise, in the NAFLD group, HDAC2, SIRT3, and VEGFA were accurate predictors (AUC > 0.7) for case and normal groups, with differential expression observed for ASS1 (p < 0.05), HDAC2 (p < 0.01), and SIRT3 and VEGFA (p < 0.001). This evidence concerns the gene ASS1 and metabolic dysfunction-associated steatotic liver disease.