The results showed that CTSG protein expression was not associated with sex, age, smoking history, lymph node metastasis and whether HIV viral RNA was detected after the first consultation (P>0.05), but was closely related to the pathological type, distant metastasis and clinical stage, with a statistically significant difference (P<0.05). The gene discussed is CTSG; the disease is metastatic malignant neoplasm in the lymph nodes.