By using the generalized linear regression with adjusted for education level, hypertension, diabetes, and hyperlipidemia, we found that the levels of plasma NfL,T-Tau, Aβ40 in normal controls were lower than that in aMCI patients, while Aβ42 and the ratio of Aβ42 to Aβ40 was higher than that in the aMCI group (p < 0.05). This evidence concerns the gene NEFL and hyperlipidemia.