Transthoracic echocardiography was performed at day 3 and 7 to measure the amount of pericardial effusion. At day 7, mice were sacrificed and hearts harvested to get the pericardium for hematoxylin and eosin staining to measure pericardial thickness and immunofluorescence and immunohistochemistry stainings to look for the 3 components of the NLRP3 inflammasome (the sensor protein NLRP3, the scaffold protein ASC, and the effector protein caspase-1). The gene discussed is CASP1; the disease is pericardial effusion.