Notably, CD276+ cells were found in eight out of nine (88.89%) ESCA lesions in the random testing of each group using immunofluorescence staining, and pre-NT, the results of immunofluorescence co-staining showed that CD16+ NK cells could recognize CD276+ tumor cells in the NCT and NICT groups (red fluorescence and green fluorescence exhibit close-range optical interference phenomenon), as shown in Figure 5C. This evidence concerns the gene CD276 and neoplasm.