The DC(I + II) exhibited significantly higher therapeutic potential than DC(I) not only toward cells but also against the metastatic HC animal model (in terms of histological investigations and tumor markers “AFP, GST, Ki-67, CSC genes, and metastasis mediators “TWIST1-induced epithelial-mesenchymal transition (EMT), and MMP9”). This evidence concerns the gene MMP9 and neoplasm.