A study in 2022 adopted target sequence capture combined with high-throughput sequencing technology to detect 6 de novo variants in such genes as HMX1, SLC4A11, TGFBI, PIKFYVE, and ZEB1 in 5 KC families, confirming that the pathogenic variants in these 5 genes are associated with the occurrence of KC (Cheng et al., 2022). This evidence concerns the gene ZEB1 and keratoconus.