Resultantly, blocking GNLY reduced the expression of cytochrome c, cleaved caspase 8, cleaved caspase 3, and cleaved PARP proteins in both cancer cell lines, where those expressions of apoptotic proteins were significantly lowered in cells treated with mNK-sEV compared to that treated with conNK-sEV (Fig. 8E–H). This evidence concerns the gene CASP8 and cancer.