As shown in Fig. S4 A–C, A2-CM-NP/siTREM2/spam1 did not obviously decrease the body weight of GBM-bearing mice, instead, the body weight of mice in IR + PD-1 inhibitor + A2-CM-NP/siTREM2/spam1 group showed a gradual increase trend after treatment (Fig. S4 C). This evidence concerns the gene PDCD1 and glioblastoma.