For instance, using CRISPR-Cas system for targeting the tumor necrosis factor receptor (TNFR) in colorectal cancer cells, established an approach for impairing proliferation and colony formation in vitro which is associated with the suppression of protein kinase B or Akt (PKB/Akt) and induction of autophagy-induced cell death (Li et al. 2023a). Here, AKT1 is linked to colorectal cancer.