Notably, the transcriptional changes observed were relatively mild, and we believe this could be explained by (1) low number of infected cells due to a low multiplicity of infection, (2) short time between the delivery of the protein and sequencing, and (3) the complexity of the regulation by MeCP2, which affects the expression of many different genes both directly and indirectly and makes the changes it mediates highly context specific with high variance31,34,58–62. Here, MECP2 is linked to infection.