Here, we applied a systematic approach on all human DUBs in the context of lung cancer metabolism, starting with a broad in silico analysis to narrow down to a DUB, ubiquitin-specific peptidase 9 X-linked (USP9x), with a potential role in regulating tumor growth via the proline metabolism pathway both in vitro and in vivo. This evidence concerns the gene USP9X and lung carcinoma.