Fourth, given the potential significantimpacts of diosgenin on ROS-NOX4 and NF-κB related mechanismsin other experimental models as indicated in the previous studies,the in vivo changes of protein expression related to the NOX4 signalingpathways were mainly evaluated for AKI and consequent CKD in the currentstudy. This evidence concerns the gene NFKB1 and chronic kidney disease.