In this present study, we firstly comprehensive verified that SPOP was downregulated expressed at transcriptional levels in ACC, BLCA, CESC, COAD, ESCA, KICH, LUAD, LUSC, OV, PRAD, READ, SKCM, STAD, TGCT, THCA, UCEC, and UCS cancers compared with para-cancer tissues, while SPOP was upregulated in DLBC, GBM, KIRC, LIHC, PAAD, and THYM, demonstrating that SPOP served as a potential biomarker in various cancers. The gene discussed is SPOP; the disease is cancer.