After treatment with XAV-939, Wnt/β-catenin pathway inhibitor, during co-culture, β-catenin was significantly decreased in HCC-LM3 cells (Fig. 3a), and TICs-related genes Oct3/4, Nanog, CD133, Sox2, c-Myc, Klf4 were down-regulated significantly compared to control (Fig. 3b). The gene discussed is KLF4; the disease is hepatocellular carcinoma.