Furthermore, we analysed the variation in the DKO, SKO, and NTC‐NTC abundance of the Raf1‐Pkm2 gene pair in cell line and tumour screening model and observed that the DKO abundance of the Raf‐Pkm2 gene pair significantly decreased in the tumour screening model, whereas it remained almost unchanged in the single knockout and control groups (Figure 3G). This evidence concerns the gene RAF1 and neoplasm.