Pathway enrichment analysis of Hepa1‐6 cells and the TCGA database suggested that double knockout or low expression of Raf1‐Pkm2 significantly disturbed fatty acid biosynthesis and ATPase‐dependent transmembrane transporter activity, both of which have been reported in previous studies to be closely associated with HCC development.31, 32, 33. This evidence concerns the gene PKM and hepatocellular carcinoma.