Additionally, further flow cytometric analysis of NADPH oxidase enzyme subunits (i.e., gp91phox, p22phox, p47phox, and p67phox) in patients and carrier mothers is useful in predicting the defective gene and enabling targeted genetic sequencing, thereby allowing for a rapid and cost-effective diagnosis of CGD (66). The gene discussed is CYBB; the disease is chronic granulomatous disease.