However, following exposure to DCIS-like tumoroids, Ob-PA and Ob-MA expressed higher levels of all studied myoCAF genes (Figure 3B): FAP (Ob-PA-T: RQ=2.69, p ≤ 0.001; Ob-MA: RQ=1.7, p ≤ 0.05) and α-SMA (Ob-PA: RQ=2.54, p ≤ 0.001; Ob-MA: RQ=1.7, p ≤ 0.05). This evidence concerns the gene ACTA1 and ductal breast carcinoma in situ.