PRF1 and infection: Across several clinical studies, the association of hemolytic E. faecalis isolated at different infection sites has demonstrated a higher prevalence of cytolysin positive E. faecalis from nonsystemic infections such as wound or urinary tract infections than from systemic infections such as bacteremia or infective endocarditis (Huycke and Gilmore 1995, Archimbaud et al. 2002, Creti et al. 2004).