To explore dysregulated gene expression in TSC, mRNA sequencing (RNAseq) data from 20 TSC patient SEN/SEGAs was compared to non-TSC brain tissue (as previously described [26]), and also RNAseq from TSC2(−) AML cells (621 – 101) was compared to TSC2(+) AML cells (621 – 103). This evidence concerns the gene TSC2 and tuberous sclerosis.