This was confirmed by STAT3 transcriptional activation ELISA, wherein the wild type Tsc2(+/+) MEF and TSC2(+) AML cells were treated with their matched TSC2-deficient cell conditioned media for 1 h, causing a large upregulation in STAT3 nuclear activation (Fig. 2c). The gene discussed is TSC2; the disease is acute myeloid leukemia.