We also knocked out ASCT2 in A498 clear cell renal cell carcinoma (ccRCC) cells, A549 and Calu6 lung cancer cell lines, and HCT116 colorectal cancer cell line and observed decreased serine uptake in all cases (Figures 2D–2G and S2B–S2E), demonstrating that ASCT2 contributes to serine uptake even in non-auxotrophic cell lines and cell lines from diverse tumor types. This evidence concerns the gene SLC1A5 and colorectal cancer.