The deficiency of GlcNAc6st2 greatly increased leukocyte infiltration in colon in DSS-induced colitis and butyrate can induce GlcNAc6st2 expression in colonic epithelial cells.33 In our work, we alsoexamined the GlcNAc6st2 expression and found IAA could similarly enhance GlcNAc6st2 expression, possibly through direct or indirect mechanisms. Here, CHST4 is linked to colitis.