To validate the effects of two prognostic-related genes, CYP24A1 and TFPI2, obtained through differential gene expression analysis in BC luminal epithelial cells and TCGA database analysis on luminal breast cancer, we first detected the expression of CYP24A1 and TFPI2 in BC tumor tissues and adjacent normal tissues using RT-qPCR and immunohistochemistry (IHC). The gene discussed is TFPI2; the disease is breast cancer.