To determine whether PRMT5 influences the radiosensitivity of solid tumor cells, U251 (glioblastoma), PSN1 (pancreatic carcinoma) and MDA-MB-231 (breast adenocarcinoma) cell lines were transfected with either non-targeted siRNA pool (siNT) or siRNA pool specific to PRMT5 (siPRMT5); 48 h after transfection cells were subjected to analyses. The gene discussed is NT5C3A; the disease is breast adenocarcinoma.