In the present study, we identified glycolysis as a key factor contributing to poor prognosis and drug resistance in RCC by using scRNA-seq analysis, and found that the glycolysis-related gene DEP domain containing 1 (DEPDC1) played a role in malignant progression and drug resistance of RCC, and may regulate RCC glycolysis through AKT/mTOR/HIF1α pathway. The gene discussed is AKT1; the disease is renal cell carcinoma.