PRF1 and neoplasm: Using isolated tumor tissue, we analyzed gene expression related to cytotoxicity (Perforin, Granzyme B, Granulysin, and FasL), inflammatory or chemotactic cytokines (IFN-γ, CXCL9, CXCL10, and CXCL11), and CD8+ T cell activation and proliferation (CD69, Tbx21, IL-2, and IL-12b).