ELISA analysis of p-ERK and p-NF-κB protein levels in MCF-7 (Figure 4F,H) and HepG2 (Figure 4G,I) cells confirmed the immunofluorescence and Western blot analysis, and showed that phosphorylated proteins were significantly reduced in cancer cells incubated with 30 μM 7-KSS for 24 h compared to the control and DMSO groups. This evidence concerns the gene MAPK1 and cancer.