CD86 and neoplasm: CD206 expression demonstrated the anti-inflammatory effect of macrophage and CD86 represented the pro-inflammatory effect of macrophage [33,34]; we found that CD206 significantly decreased while CD86 showed no change in the spleen from experiment-grouped mice (Figure 5D and Figure S1E), indicating that α-LNPs facilitated the anti-tumor response of the tumor-burdened mice.