In the DS tuberculosis model, double immunization with TB/FLU-06E at the start of the HR therapy resulted in a significantly higher level of total spleen-derived cytokine-producing CD4+ and CD8+ Tem cells (CD44+ CD62L-), with a markedly higher proportion of IFN-γ and IFN-γ,TNF-α producing cells. The gene discussed is CD8A; the disease is tuberculosis.