This was recapitulated in a mouse model of diethylnitrosamine (DEN) plus carbon tetrachloride (CCl4)-induced HCC, as animals with nonfunctional TLR-4 (TLR4-mut) had a significant reduction in tumor number and size but no difference in tumor incidence when compared to TLR-4 wild-type mice exposed to the same oncogenic toxins [37]. Here, TLR4 is linked to neoplasm.