CancerSEEK evaluates for mutations in 61 regions of 16 cancer-driver genes (AKT1, APC, BRAF, CDKN2A, CTNNB1, EGFR, FBXW7, FGFR2, GNAS, HRAS, KRAS, NRAS, PIK3CA, PPP2R1A, PTEN, and TP53) and measures the levels of eight cancer-associated protein biomarkers (CA19-9, CA-125, CEA, HGF, MPO, OPN, PRL, and TIMP-1) [27]. This evidence concerns the gene SPP1 and cancer.