Otherwise, the levels of atrophy markers, FBXO32 and TRIM63 (also known as Atrogin-1 and MURF1), in skeletal muscle were detected and analyzed by RT-qPCR and IHC, and it was found that FBXO32 and TRIM63 increased significantly in the T2DM group but partially attenuated in the GFP-P group (Figure 6b–e), indicating that GFP could prevent skeletal muscle atrophy in T2DM rats. The gene discussed is TRIM63; the disease is type 2 diabetes mellitus.