HFD-induced obesity in C57BL/6 mice resulted in ER stress, intracellular free cholesterol accumulation, lipogenesis, NF-κB signaling, and a proinflammatory secretion profile of adipokines (resistin, TNF-α) in hypertrophic adipocytes, as well as systemic dyslipidemia and hyperglycemia [78,79]. This evidence concerns the gene TNF and obesity due to melanocortin 4 receptor deficiency.