Upregulation of adiponectin and SIRT-1 expression after sleeve gastrectomy in a murine model of sequential HFD-induced obesity and streptozocin-induced T2DM resulted in browning of SAT via PPARγ, PGC-1α, and UCP1 activation [293]. This evidence concerns the gene ADIPOQ and obesity due to melanocortin 4 receptor deficiency.