The hypermethylation of the CpG site in the CTCF region upstream of the PDGFRA gene locus, caused by the increased concentration of 2-HG, contributes to a decrease in the binding strength of CTCF to DNA, thereby disrupting the TAD structure, which ultimately leads to the incorrect interaction of the enhancer with the PDGFRA promoter, leading to the upregulation of the oncogene in AML cells with an IDH1R132H mutation [12,28]. Here, CTCF is linked to acute myeloid leukemia.