Towards this goal, we present the results of the performance evaluation of a novel automated real-time qPCR cartridge-based NPM1 mutation assay (Xpert NPM1 mutation assay) for the quantification of mutant NPM1 mRNA transcripts (types A, B, and D in exon 12) using peripheral blood samples from NPM1-mutated AML patients. This evidence concerns the gene NPM1 and acute myeloid leukemia.