The identification of BRAF p.(Val600Glu) and KRAS p.(Gly12Cys) variants in two of those samples by NGS provided indirect evidence that the qPCR result could have been wrong due to general mutual exclusivity of class 1 BRAF mutations, KRAS codon 12 variants and activating EGFR mutations in NSCLC [32,33]. The gene discussed is EGFR; the disease is non-small cell lung carcinoma.