Western immunoblot analysis of Akt and AMPK proteins expression in breast cancer cells (MCF-7 and MDA-MB-231) exposed (for 24 h) to the EDAG-1 and EDAG-8 derivatives and cisplatin (0.5 and 1 μM) also confirmed, as before, the results obtained using the cytometric method (Figure 18; for original membrane images from the Western blotting, see the Supplementary Materials—Figures S5–S7). The gene discussed is AKT1; the disease is breast carcinoma.