Hence, in addition to investigating the levels of Akt, to confirm the test of autophagy induction, we undertook the analysis of AMPK kinase expression upon exposure to the compounds in the study (EDAG-1 and EDAG-8) and cisplatin (treatment duration: 24 h; concentrations of 0.5 and 1 μM) in breast cancer cells (MCF-7 and MDA-MB-231). This evidence concerns the gene AKT1 and breast carcinoma.