The results revealed that, compared to the ND group, the HFD group exhibited conspicuous co-localization of α-SMA+CD68+KLF4+, α-SMA+CD68+p-JAK2+, and α-SMA+CD68+p-STAT3+ at the foam cell aggregation regions of atherosclerosis plaque-rich aortic. The gene discussed is STAT3; the disease is atherosclerosis.