Looking for a prognostic role of HIPK2 in colon cancer, the authors analyzed a retrospective series of 80 primary patients’ samples at different tumor stage of colon cancer and normal mucosa samples (taken at distance from the tumor) by immunofluorescence and tissue microarray (TMA) by using multiplexed tissue cytometry capable of exploring correlative protein expression at the single tumor cell level on TMA, simultaneously monitoring HIPK2 and p53 protein expression at the single cell level. This evidence concerns the gene TP53 and malignant colon neoplasm.