Variants that exclusively appeared in the primary or recurrent samples (i.e., APC, NOTCH1 in GBM-P and PTPN11, BAP1, STK11 and NF1 in GBM-R) probably are signs of branching clonal evolution patterns influenced, at least in part, by treatment effects [14], while the detection of shared variants aligned more with the linear evolution pattern (i.e., PTEN, EGFR, TP53). The gene discussed is STK11; the disease is glioblastoma.