In this study, the results showed that a uterine infusion of 50 μL LPS (1 mg/mL) significantly increased TNF-α, IL-6, and IL-1β levels in the uterine tissue of mice, whereas 30 mg/kg leonurine significantly reduced their levels in LPS-induced mouse uterine tissue, suggesting that leonurine may exert anti-inflammatory effects on LPS-induced endometritis by inhibiting proinflammatory cytokine release. The gene discussed is TNF; the disease is endometritis.