Results of immunoprecipitation and BiFC assays showed that overexpression of ajuba LIM protein (AJUBA) can recruit tumor necrosis factor associated factor 6 (TRAF6), enhance Akt phosphorylation, activate the Akt/GSK-3β/Snail signaling pathway, and promote the EMT process, thereby enhancing the invasion and metastasis ability of HCC (Hepatocellular Carcinoma cells) [118] (Figure 3B). This evidence concerns the gene AKT1 and hepatocellular carcinoma.