In order to explore the involvement of the DS variant-mediated oxidative stress in the activation of MLKL by these molecules, we used immunofluorescence to measure the level of phospho-MLKL in the breast cancer cells that were exposed to these glycans with or without NACA, which is a universal antioxidant agent [22] (Supplementary Figure S4). The gene discussed is MLKL; the disease is breast cancer.